Sperm DNA fragmentation: laboratory and clinical aspects

Sperm DNA fragmentation: laboratory and clinical aspects

Clinical aspects of sperm DNA fragmentation detection and male infertility.

Over the past 25 years, various methods have been developed to measure sperm DNA strand breaks in situ. Currently, there are four major tests of sperm DNA fragmentation, including the Comet, Tunel, sperm chromatin structure assay (SCSA) and the acridine orange test (AOT). The Comet assay is a light microscope technique where the sperm cells are mixed with melted agarose and then placed on a gla...

Sperm DNA fragmentation in clinical practice

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. We value the comments written by Dr. Benagiano on the “Clinical utility of sperm DNA fragmentation testing: practice recommendations based on clinical scenarios” (1). The author has pointed out the controversies surrounding the diagnostic potential of conventional semen parameters and tests of sperm DNA fragmentation ...

Clinical usefulness of sperm DNA fragmentation testing

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. The search for the best biomarker for the diagnosis of male infertility is more evident in recent years as conventional semen analysis is not always an optimal predictor of male fertility. Since the advent of the intra-cytoplasmic sperm injection (ICSI) for male factor infertility, the pace of research related to sper...

Sperm DNA fragmentation testing—clinical utility

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. The expert panel made a tremendous effort to propose guidelines on the emerging trends of sperm DNA fragmentation testing and its clinical utility based on the available latest scientific evidence (1). Panel presented it in a systematic manner by a practical approach with four common clinical case scenarios. In the er...